Britain's Dan Evans saw his hopes of making the Wimbledon first round extinguished as he fell at the last hurdle of qualifying against Japan's Yuichi Sugita.
Evans has made the first round at SW19 three times before in his career but Sugita, ranked 149th in the world, proved too strong and cruised to a 7-6 (8/6) 6-2 7-5 victory.
It means no British male qualifiers will join Friday's main draw after Richard Gabb, Ed Corrie, Daniel Cox, Joshua Milton, Alexander Ward and Richard Bloomfield had also lost in earlier rounds.
Evans fought back from 3-0 down in the first to lead Sugita 5-3 at Roehampton but the Briton failed to serve out and his opponent took full advantage.
A tight first set was followed by a comfortable second for Sugita, who went on to book his place in the main draw for the first time in two hours and 13 minutes.
Britain will have six men in the first round with Andy Murray leading the charge along with Aljaz Bedene while Kyle Edmund, James Ward, Liam Broady and Brydan Klein have been given wild cards.
In the women's tournament, Heather Watson took her place by right and wild cards have also been handed to Laura Robson, Johanna Konta and Naomi Broady.
Source: PA
