Jose Acasuso turned professional in 2000, playing futures and challenger events. In 2001, he made an immediate impact in his first ATP tournament in Buenos Aires, where he defeated former No. 10 player Felix Mantilla in the last round of the qualifying to make the main draw, and then defeated compatriots Franco Squillari in the quarter-finals and Gaston Gaudio in the semi-finals.
Acasuso is known for his strong serve and his hard groundstrokes off both sides.
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